Identification of new DNA i-motif binding ligands through a fluorescent intercalator displacement assay
نویسندگان
چکیده
منابع مشابه
A fluorescent intercalator displacement assay for establishing DNA binding selectivity and affinity.
A summary of the qualitative and quantitative elements of a fluorescent intercalator displacement (FID) assay useful for establishing the DNA binding selectivity, affinity, stoichiometry, and binding site size and distinguishing modes of DNA binding is provided.
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متن کاملIdentification of new DNA i-motif binding ligands through a fluorescent intercalator displacement assay† †Electronic supplementary information (ESI) available: Experimental, supporting fluorescence and UV-Vis data, Job plot, binding curves, CD spectra, melting temperatures and SPR data. See DOI: 10.1039/c7ob00710h
i-Motifs are quadruplex DNA structures formed from sequences rich in cytosine and held together by intercalated, hemi-protonated cytosine-cytosine base pairs. These sequences are prevalent in gene promoter regions and may play a role in gene transcription. Targeting these structures with ligands could provide a novel way to target genetic disease but there are very few ligands which have been s...
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ژورنال
عنوان ژورنال: Organic & Biomolecular Chemistry
سال: 2017
ISSN: 1477-0520,1477-0539
DOI: 10.1039/c7ob00710h